Reorganized flat 41-file directory into structured layout with: - scripts/ for Python analysis code with shared config.py - notebooks/ for Jupyter analysis notebooks - data/ split into raw/, metadata/, processed/ - docs/ with analysis summary, experimental design, and bimodal hypothesis tutorial - tasks/ with todo checklist and lessons learned - Comprehensive README, PLANNING.md, and .gitignore Co-Authored-By: Claude Opus 4.6 <noreply@anthropic.com>
68 lines
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2.6 KiB
Markdown
68 lines
No EOL
2.6 KiB
Markdown
# Analysis Summary: Distance Between Flies
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## Key Findings
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### Overall Statistics
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- Trained flies (aligned to barrier opening):
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- Data points: 46,613
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- Mean distance: 92.72
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- Std distance: 111.86
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- Untrained flies (aligned to barrier opening):
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- Data points: 46,866
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- Mean distance: 78.67
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- Std distance: 102.86
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### Pre-Opening Period (t < 0)
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During this period, flies are physically separated by a barrier:
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- Trained mean distance: 155.27
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- Untrained mean distance: 147.19
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### Post-Opening Period (t > 0)
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After the barrier is removed and flies can interact:
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- Trained mean distance: 77.44
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- Untrained mean distance: 61.87
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## Statistical Tests
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### Pre-opening period comparison:
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- Trained mean: 155.27, Untrained mean: 147.19
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- T-statistic: 4.4024, P-value: 1.08e-05
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- Cohen's d: 0.0654
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### Post-opening period comparison:
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- Trained mean: 72.52, Untrained mean: 63.86
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- T-statistic: 30.4693, P-value: 1.08e-203
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- Cohen's d: 0.0914
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### Within-group changes:
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- Trained flies - Pre vs Post:
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- Mean change: -82.75
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- T-statistic: 77.2805, P-value: 0.00e+00
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- Cohen's d: -0.8313
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- Untrained flies - Pre vs Post:
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- Mean change: -83.33
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- T-statistic: 84.4719, P-value: 0.00e+00
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- Cohen's d: -0.9022
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## Interpretation
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1. **Pre-opening**: Both groups show high distances as expected since flies are separated by a barrier. There is a statistically significant difference between groups (p < 0.001), but the effect size is very small (Cohen's d = 0.0654).
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2. **Post-opening**: Both groups show reduced distances after the barrier is removed, indicating interaction between flies. There is a statistically significant difference between groups (p < 0.001) with a small effect size (Cohen's d = 0.0914).
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3. **Within-group changes**: Both trained and untrained flies show significant decreases in distance after the barrier opens (p < 0.001). The effect sizes are large for both groups (Cohen's d = -0.8313 for trained, -0.9022 for untrained).
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4. **Group Differences**:
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- Trained flies maintain slightly higher distances than untrained flies both before and after barrier opening.
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- This could indicate different behavioral patterns between trained and untrained flies, though the effect sizes are small.
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## Visualization
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The plot "avg_distance_aligned_to_opening.png" shows:
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- Blue line: Average distance for trained flies (smoothed)
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- Red line: Average distance for untrained flies (smoothed)
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- Black dashed line: Time of barrier opening (t=0)
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The visualization clearly shows the transition from high distances (pre-opening) to lower distances (post-opening) for both groups, with trained flies consistently maintaining slightly higher distances than untrained flies. |